Steam Sterilization is common in the Life Sciences industry and is generally executed in Autoclaves. Steam under pressure is used to kill micro-organisms; the required time and pressure will vary based on the items being sterilized and the type of Autoclave used. Common autoclave cycles include gravity, pre-vacuum and/or post vacuum, liquids and immediate use/flash. Below are common challenges, important considerations and Crest's solutions for autoclave Temperature mapping qualifications.